Roles of integrin in tumor development and the target inhibitors / 中国天然药物

Integrin is a large family of cell adhesion molecules (CAMs) which involves in the interaction of cells/cells and cells/ extracellular matrix (ECM) to mediate cell proliferation, differentiation, adhesion, migration, etc. In recent years, aberrant expression of integrin has been clearly found in many tumor studies, indicating that integrin is closely related to tumor formation and development. Meanwhile, it has effects on tumor cell differentiation, cell migration, proliferation and tumor neovascularization. The study of drugs targeting integrins is of great significance for the clinical treatment of tumors. Because of its important role in tumorigenesis and development, integrin has become a promising target for the treatment of cancer. This review summarizes the role of integrin in tumor development and the current state of integrin inhibitors to provide a valuable reference for subsequent research.

PFKFB3 promotes cell proliferation, migration, invasion and metastasis in adenoid cystic carcinoma ACC-2 cells / 国际肿瘤学杂志

Objective To evaluate the expression of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) in adenoid cystic carcinoma and the effects of PFKFB3 on the proliferation,migration,invasion and tumorigenesis in vivo of adenoid cystic carcinoma cells.Methods Immunohistochemistry and Westem blotting were applied to detect the expression of PFKFB3 in 29 cases of adenoid cystic carcinoma tissues and 10 cases of para-carcinoma normal salivary gland tissues.PFKFB3 in adenoid cystic carcinoma ACC-2 cells was silenced by adenovirus vector.The effects of PFKFB3 silence on cell proliferation,migration,invasion and tumorigenesis in vivo and distant metastasis of adenoid cystic carcinoma cells were evaluated by cell proliferation assay,wound healing assay,Transwell assay,xenografted mice model and lung metastasis mice model.Results The results of immunohistochemical staining showed that the positive expression rate of PFKFB3 in adenoid cystic carcinoma tissues was 93.1％ (27/29),and the positive expression rate of PFKFB3 in normal salivary gland tissues was 20.0％ (2/10),with a significant difference (x2 =20.84,P ＜ 0.001).The results of methyl thiasolyl tetrazolium (MTT) assay showed that,compared with ACC-2 group,the proliferation activity of cancer cells with silence of PFKFB3 (PFKFB3--ACC-2) was significantly suppressed for 72 h (1.8 ± 0.2 vs.4.7 ± 0.8,t =3.582,P =0.001).The results of wound healing assay showed that after scraping cells away for 24 h,the number of cells in the scratch area was 99.8 ± 13.2 in the PFKFB3--ACC-2 group,which was significantly less than that in the ACC-2 group (263.0 ± 97.4,t =2.868,P =0.029).Transwell results indicated that the number of cells passing through matrigel was 17.6 ± 2.1 in the PFKFB3--ACC-2 group,which was less than that in the ACC-2 group (28.6 ± 3.8,t =4.452,P =0.004).The tumor volume in the PFKFB3--ACC-2 [(623.5 ± 134.1) mm3] was smaller than that in the ACC-2 group [(1 621.0 ±278.1) mm3,t =4.213,P =0.001].More pulmonary metastases were found in the ACC-2 group thanPFKFB3--ACC-2group(18.1±3.2vs.4.1±2.2,t=6.322,P=0.001).Condusion The expression of PFKFB3 is higher in adenoid cystic carcinoma tissue than normal salivary gland tissue,and the highly expressed PFKFB3 plays a driving role in the proliferation,migration,invasion and metastasis in adenoid cystic carcinoma.

Function of SIRT6 in tumor initiation and progression / 生物工程学报

As a member of the sirtuins family, also called Class III histone deacetylases (HDACs), SIRT6 has many catalytic enzyme activities and plays a pivotal role in biological processes including anti-aging, chromatin regulation, transcriptional control, glucose and lipid metabolism, and DNA damage repair. Recently, increasing evidences indicated that SIRT6 was related to initiation and development of tumors, such as hepatic cancer, lung cancer, breast cancer and genital system tumors. However, SIRT6 might play a dual role in tumorigenesis and progression. SIRT6 often acted as a tumor suppressor, but might play an oncogenic role. Based on our current study, we depicted the essential roles of SIRT6 in the initiation and progression of various tumors, and summarized its mode of actions, which might provide clues for cancer therapy.

Ezrin protein and its importance in tumor / 肿瘤研究与临床

Ezrin protein function as molecular linker between the actin-cytoskeleton and the plasma membrane,which involves in the formation of microvilli and the maintenance of cello-morphology, movement, adhereance as well as survival, intimate studies of its structure and function have been done. At present many studies indicate that Ezrin involves in the tumor development and tumor metastasis,relate to prognosis. To study Ezrin protein in tumor will provide us other way and standard for diagnose and treatment of tumor and prognostic evaluation.

Expression of Chemokine Receptors in Ovarian Cancer Tissue / 대한산부인과학회잡지

OBJECTIVE: We investigated the expression of chemokine receptors in human ovarian cancer to understand the role of chemokines in ovarian cancer development and metastasis. METHODS: Twenty-two cases of epithelial ovarian cancer were studied for expression of 13 chemokine receptors such as CXCR1-CXCR5 and CCR1-CCR8 by using semi- quantitative RT-PCR and immunohistochemistry. Moreover, we studied the relationship between the chemokine receptors expression and lymph nodes metastasis of ovarian cancers. RESULTS: As compared with normal ovarian tissues, ovarian cancer tissues showed higher mean expression levels of CCR1,3,4,5,7,8 and CXCR1,3,4. Of chemokine receptors, CCR7 revealed the significantly higher levels of expression in ovarian cancer tissues relative to normal tissues. In the cases of retroperitoneal lymph nodes metastasis, increased expression of CCR2,4 and CXCR 1,3,4 was observed although there was no statistical significance. CONCLUSION: These results suggest that there is a complex chemokine/chemokine receptor network in pathogenesis and the way of lymph node metastasis of ovarian cancer rather than a specific chemokine or chemokine receptor.